Application

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

The MISSION TRC2 Control Vector pLKO-puro TurboGFP is an 8802 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the TRC2 MISSION shRNA library clones. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP Control Vector is provided as 10 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/µl.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

TurboGFP is a trademark of Evrogen Co.